• lab-217043_1280

Ego ole ka a na-agbakwunye mmiri na cell shaker

Na omenala nkwusioru cell,cell shake flaskbụ ụdị omenala cell eri eri.Uto nke sel kwụsịtụrụ adabereghị n'elu ihe na-akwado ya ma na-eto eto na nkwụsịtụ na omenala omenala.Kedu ka anyị ga-esi chọpụta oke mmiri mmiri a ga-agbakwunye na omenala n'ezie?

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Nkọwapụta nke cell shaker na-agụnye 125ml, 250ml, 500ml na 1000ml iji gboo mkpa dị iche iche nke omenala cell.Dịka ọmụmaatụ, a na-ejikarị karama 125ml na 250ml nwere obere ikike maka obere nyocha, ebe a na-eji nkọwa 500ml na 1000ml maka nyocha omenala cell nke ọkara.Mgbe ị na-eji ụdị ihe oriri a, a ga-eji vibration nke shaker mee ihe iji belata ọnụ ọgụgụ cell agglomeration ma nọgide na-enwe ọganihu dị mma nke mkpụrụ ndụ.Omenala cell kwesịrị ime ya na gburugburu ebe adịghị mma.Ya mere, a ga-eme ka flask omenala triangle pụrụ iche tupu etinye ya n'ọrụ iji nweta mmetụta nke enweghị DNAase, enweghị enzyme RNA, na enweghị ihe ndị sitere n'anụmanụ, na-enye ebe dị mma maka uto cell.

Dị ka nkọwa dị iche iche nke karama ahụ si dị, a na-atụ aro ndochi nke nkọwa anọ nke karama ahụ site na ala ruo elu bụ 30ml, 60ml, 125ml, 500ml.N'ozuzu, a na-achịkwa olu nke ngwọta na omenala nke mkpụrụ ndụ ihe dịka 20% -30% nke ngụkọta nke karama ahụ na-ama jijiji, na e nwere akara ngosi doro anya na karama ahụ iji kwado nlele anya nke ike nke ngwọta. .

Ihe dị n'elu bụ ọnụọgụ mmiri akwadoro agbakwunyere na nkọwapụta dị iche iche nke cell shaker, nke na-adịghị edozi.Ekwesịrị ịtụle ikike ahụ nke ọma dịka njirimara nke uto cell na njupụta inoculation, iji zere mmetụta nke uto cell n'ihi oke mmiri mmiri agbakwunyere.


Oge nzipu: Sep-27-2022