Enwere ọtụtụ iberibe cell mgbe arụ ọrụ lysis nke cell nkwusioru.Kedu ka esi ewepu iberibe ndị a?Ka anyị leba anya n'ụzọ dị iche iche:

1. Jiri usoro dilution.Mgbe a na-agbaze mkpụrụ ndụ ndị ahụ, ha nwekwara ike ịba ụba, ha ga-abawanye ụba, na irighiri mkpụrụ ndụ ga-adịkwa ntakịrị.
2. Enwekwara mmezi eke.Selụ na-edozi ngwa ngwa karịa ọtụtụ iberibe: Bugharịa nkwusioru cell n'imecentrifuge tube, na mgbe ọtụtụ n'ime sel ndị ahụ dara, enwere ike ịmịpụta ihe ngwọta nke elu, wee tinye ya na ngwọta omenala iji kwụsịtụ sel.Enwere ike iji usoro a ugboro ugboro, ebe oge ọ bụla ị nwere ike ịhụ ma ọ na-emezu ihe ị chọrọ n'okpuru microscope.
3. centrifuge dị alanwere ike iwepu irighiri ihe, n'ozuzu 700g,5mins
4. Mgbe centrifu, n'okpuru ọnọdụ na ọnụ ọgụgụ nke mkpụrụ ndụ zuru ezu, belata oge centrifugation, dị ka 5min, 1000rpm, kama 3min, 1000rpm, ma wepụ ihe dị elu, n'ihi na necrosis na irighiri ihe na-adịkarị na elu!A na-arụ ọrụ a naanị otu ugboro n'oge usoro ịsa ahụ tupu ịmalite!

Ọ bụ ezie na e nwere ọtụtụ ụzọ isi wepụ ihe mkpofu cell, ị ga-achọpụta na centrifugation bụ otu n'ime ụzọ kachasị mma!

Na n'ihi na ihe ọkụkụ na sel nwere mgbidi cell nke nwere cellulose, hemicellulose na pectin, ọ dị mkpa ka a na-egweri ájá quartz ma ọ bụ ntụ ntụ na ngwọta kwesịrị ekwesị ma ọ bụ na-emeso ya na cellulose iji nweta nzube ahụ.Nkewa nke nje bacteria na-esiwanye ike karị, n'ihi na ọkpụkpụ mgbidi cell nke nje bacteria bụ n'ezie njikọ nke peptidoglycan cystic macromolecules, siri ike.Ụzọ ndị a na-ejikarị agbaji mgbidi sel nje na-agụnye ultrasonic ngwepịa, egweri ájá, extrusion elu ma ọ bụ ọgwụgwọ lysozyme.Mgbe anụ ahụ na sel gbajiri, a na-ahọrọ ihe nchekwa kwesịrị ekwesị iji wepụ protein a chọrọ.A na-ewepụ ihe ndị na-adịghị edozi ahụ dị ka iberibe cellcentrifugema ọ bụ filtration.

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