Hichapụ omenala flaskbụ na ogbo nke nsogbu screening na omenala (pilot ule), omenala ọnọdụ kwesịrị ịdị nso dị ka o kwere mee ka gbaa ụka mmepụta omenala ọnọdụ, ọrụ ibu ibu, ogologo oge, mgbagwoju ọrụ.Ihe ndị na-emetụta elu arụmọrụ nke ịma jijiji flask omenala bụ tumadi omenala okpomọkụ, n'ikwe amplitude nke shaker, ego nke ịma jijiji flask, na pH nke omenala ọkara, na viscosity nke ọkara, wdg Culture okpomọkụ: mycelium ibu okpomọkụ: na mycelium ibu okpomọkụ. nke dị iche iche oriri fungi dịkwa iche iche, ọtụtụ ndị kwesịrị ekwesị ibu okpomọkụ dị n'etiti 22 ℃ na 30 ℃, ma ọ bụrụ na omenala okpomọkụ dị oke ala, mycelium ibu bụ ngwa ngwa;Mgbe okpomọkụ dị elu nke ukwuu, pellet mycelium adịchaghị mma ma dị obere, ike na ịdị mma nke pellet mycelium belatara.
N'ikwe ugboro na elu arụmọrụ n'ikwe karama loading: oriri fungi bụ aerobic dịkwa ka usoro ha, mmiri mmiri omenala, tumadi site absorption nke oxygen etisasịwo na omenala usoro.A na-emetụta ikuku oxygen gbazere na omenala omenala site na viscosity nke ọkara, ọnụ ọgụgụ nke mmiri mmiri na akpa, ugboro nke oscillation na ihe ndị ọzọ.Ugboro ugboro na-ama jijiji dị ukwuu, flask na-ama jijiji dị ntakịrị, ntinye uche nke ọkara dị elu, ikuku oxygen gbazere nke ọkara dị elu, na ụzọ ọzọ dị ala.Na-emekarị rotary shaker ọsọ bụ 180-220 RPM / min, reciprocating bụ 80-120 RPM / min, njupụta 6-7cm.
Ph nke usoro ọdịnala: PH nke usoro ọdịnala na-emetụta nnabata nri, ọrụ enzyme na uto mycelial pellet.A ga-edozirịrị pH akọwapụtara tupu igba ogwu, ọtụtụ fungi a na-eri na pH 2.0-6.0.Iji gbochie mgbanwe dị ukwuu nke PH na omenala omenala, a na-agbakwunye calcium carbonate, phosphate na ihe ndị ọzọ na-echekwa ihe na omenala omenala.
Ọkara viscosity: Ọkara viscosity na-emetụta kpọmkwem ọnụọgụ oxygen gbazere na ya, ma na-emetụtakwa nhazi nke pellets mycelial.Nsonaazụ gosiri na mgbe viscosity nke omenala omenala mụbara, dayameta nke mycelium pellets belatara, ọnụ ọgụgụ na-abawanye, na mkpụrụ na-abawanye.Ya mere, dị ka ihe chọrọ nke mmiri mmiri nsogbu na dayameta nke mycelium pellets, omenala usoro nwere ụfọdụ viscosity kwesịrị ahazi.Omenala sel bụ ọrụ siri ike, ọkachasị mgbe ọ dị mkpa ka emebe ya site n'enyemaka nke onye na-ama jijiji, dị ka ihe na-eme ka ọ dị elu, a ga-atụle ya nke ọma, iji hụ na ọ na-aga nke ọma nke ọdịbendị sel.
Oge nzipu: Ọktoba 24-2022