Udi mmiri bụ ụdị nke ejiri usoro omenala mmiri mebere.Ọ nwere uru nke mkpụmkpụ mmepụta okirikiri, nje nje afọ na-agbanwe agbanwe, adaba inoculation, adabara factory mmepụta, na na.Ọtụtụ ndị na-eto eto anabatala ya.Cell shaker (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/） bụ ụdị ihe eji eme ihe dị mkpa na omenala nke ụdị mmiri mmiri.Isi ihe ndia kwesiri ka elebara anya na omenala:

1.Preparation nke omenala usoro dị ka usoro nke dị iche iche mmiri, n'ime akpa, igbari na mmiri, kewara triangular karama, n'ozuzu 250 ~ 300mL cell shake karama 50mL omenala ọkara,500 ml nke triangle  (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/）100ml omenala ọkara, nkwụnye owu plọg, na akwụkwọ akụkọ ọbọp;Enwere ike iji akwa mkpuchi 8 nke gauze 8cm × 8cm mechie mkpuchi karama, n'èzí na-eji akwụkwọ agba aja aja akara.
2. Autoclave sterilization usoro bụ n'ozuzu nakweere maka igba ogwu, nke na-achọ 121 ℃ na 30min.Wepụ ya ma kpoo ya ihe dịka 30 ℃.Tinye ya n'ime ụlọ na-adịghị mma ma ọ bụ igbe inoculation maka ojiji.
3. Dị ka aseptic ọrụ chọrọ, onye ọ bụla karama nwere ike jikọọ na a 2 ~ 3cm2 slant strain, na onye ọ bụla slant strain nwere ike jikọọ na 4 ~ 5 karama.Ọ ka mma iweta ụfọdụ ndị na-emepụta ihe na-agbakwunyere, ka ha wee na-ese n'elu elu nke ọkara.Mgbe inoculation gasịrị, kpuchie ọnụ karama ahụ na gauze nke etinyere na mbụ, ma kee ya nke ọma na eriri.
4. Enwere ike itinye karama eriri inoculated n'ime akwa na-ama jijiji maka ọdịbendị, ma ọ bụ tinye ya na okpomọkụ nke 24 ~ 26 ℃ maka 48h, wee mee omenala oscillated mgbe aerogenic mycelium gbatịchara n'ime usoro omenala. .Ugboro ugboro nke shaker na-emegharịgharị bụ 80 ~ 120r/min, na nke rotary shaker bụ 150 ~ 220r/min.Enwere ike ịzụlite 3 ~ 4d na shaker.Na njedebe nke omenala, n'ihi ụdị dị iche iche, omenala omenala pụtara dị iche iche na agba, dị ka oporo ero, enoki ero omenala ọkara bụ ìhè edo edo;Mushroom, ericius erinaceus omenala onye na-acha uhie uhie na-acha uhie uhie, na ísì ero;Omenala ero ero bụ agba aja aja, oke na ụtọ.Enwere ike iji usoro omenala maka mmepụta ma ọ bụ ọdịbendị mgbasawanye ọzọ naanị mgbe ịdị arọ nke mycelium ruru 10g / L na dayameta nke pellets mycelium bụ 1 ~ 2mm.
 
5. Nke abụọ mmiri mmiri nsogbu mmepụta nke abụọ mmiri mmiri nje omenala ọkara mmepụta nke otu larịị nke mmiri mmiri nsogbu, omenala akpa kwesịrị ibu, ike bụghị ihe karịrị 3500mL.Mgbe ịgba ọgwụ mgbochi ahụ na jụrụ oyi, a na-etinye ụdị mmiri mmiri mbụ fermented n'ime ya5L cell shaker(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/）na oke nke 5% -10% na omenala na shaker na ngwa ngwa ngwa ngwa.Mgbe oge ụfọdụ nke omenala oscillating gachara, enwere ike nweta ụdị mmiri mmiri na nkesa nke microspheres na mmiri ịgba mmiri doro anya.
Na nchịkọta, mgbe ị na-eji cell shaker na omenala ụdị mmiri mmiri, rụọ ọrụ dị ka usoro ndị a dị n'elu, ma ṅaa ntị iji chịkwaa okpomọkụ, ugboro ugboro na ihe ndị ọzọ na usoro iji hụ na mmetụta omenala.
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