Ozugbo sel anyị na-emepe emepe

ụlọ ọrụ cellbụ ndị mmeru, ọtụtụ n'ime ha na-esi ike ijikwa.Ọ bụrụ na mkpụrụ ndụ emetọọ bara uru ma sie ike inweta ọzọ, enwere ike iji ụzọ ndị a wepụ ha.

1. Jiri ọgwụ nje mee ihe

Ọgwụ nje na-adị irè karị n'igbu nje bacteriaụlọ ọrụ cell.Ọgwụ ngwakọta dị irè karịa ọgwụ naanị.Ọgwụ mgbochi dị irè karịa ọgwụ mgbe mmetọ gasịrị.Ọgwụ mgbochi na-ejikarị ọgwụ nje abụọ (penicillin 100u/ml tinyere streptomycin 100μg/mL).Mgbe mmetọ ahụ gasịrị, usoro nhicha kwesịrị ịdị okpukpu 5 ruo 10 karịa ego a na-emebu.Ekwesịrị iji ọgwụ ahụ maka awa 24 ruo 48 mgbe mgbakwunye, wee dochie ya na usoro a na-emebu.Mmiri omenala.Usoro a nwere ike ịdị irè na mmalite nke mmetọ.Na mgbakwunye na penicillin na streptomycin, ọgwụ nje ndị a na-eji nwekwara ike ịgụnye gentamicin, kanamycin, polymyxin, tetracycline, nystatin, wdg. A na-ejikarị 400 ruo 800 μg/mL kanamycin ma ọ bụ 200 μg/mL tetracycline.A na-agbanwe usoro ahụ kwa ụbọchị 2 ruo 3 ma nyefee ya maka ọgbọ 1 ruo 2 maka ọgwụgwọ.N'ime afọ ndị na-adịbeghị anya, a kọwapụtara na 4-fluoro, 2-hydroxyquinoline (Ciprofloxacin, Cip), Pleu-romutilin derivative (Pleu-romutilin derivative, BM-Cyclin2: BM-1 na tetracycline derivative (BM-2)) bụ ọgwụ nje. dị irè na igbu mycoplasma mgbe ejiri ya naanị ma ọ bụ na ngwakọta.A na-akwado ọgwụ nje atọ ndị a n'ime 250X ngwọta etinyere na PBS ma chekwaa na -20 Celsius maka iji emechaa.Nleta ojiji nke Cip bụ 10 μg/mL, BM-1 bụ 10 μg/mL, na BM-2 bụ 5μg/mL.Mgbe ị na-eji, buru ụzọ mee ka usoro omenala metọọ, tinye RPMI1640 omenala nwere BM-1, wee chọọ usoro omenala mgbe ụbọchị 3 gachara, tinye RPMI1640 omenala nwere BM-2, na omenala maka ụbọchị 4, na ihe ndị ọzọ maka ụbọchị 3 n'usoro. .gburugburu, ruo mgbe 33258 fluorescent staining microscopy gosipụtara na e kpochapụrụ mycoplasma, mgbe ahụ, a na-agbakwunye omenala omenala na oge 3-4.

2. ọgwụgwọ ikpo ọkụ

Ịbanye omenala anụ ahụ emetọọ na 41 Celsius C maka awa 18 nwere ike igbu mycoplasma, mana ọ nwere mmetụta ọjọọ na sel.Ya mere, a ga-eme nyocha mbụ tupu ọgwụgwọ iji chọpụta oge ọkụ nke nwere ike igbu mycoplasma ruo n'ókè kachasị ma nwee mmetụta kachasị na sel.Usoro a mgbe ụfọdụ enweghị ntụkwasị obi.Ọ bụrụ na a na-eji ọgwụ eme ihe na mbụ wee kpoo ọkụ na 41 Celsius, mmetụta ahụ ga-aka mma.

3. Jiri mycoplasma-kpọmkwem serum

Enwere ike wepu mmetọ mycoplasma site na 5% oke bekee mycoplasma mgbochi serum (hemagglutination titer 1:320 ma ọ bụ karịa).N'ihi na ọgwụ mgbochi a kapịrị ọnụ nwere ike igbochi uto nke mycoplasma, ọ na-atụgharị na-adịghị mma ụbọchị 11 mgbe ọgwụgwọ antiserum gachara ma na-anọgide na-adịghị mma mgbe ọnwa 5 gachara.adịghị mma.Otú ọ dị, usoro a na-enye nsogbu karị ma ọ bụghị dị ka ọ dị mma na nke akụ na ụba dị ka iji ọgwụ nje mee ihe.

4. Ụzọ ndị ọzọ

Na mgbakwunye na ụzọ ndị a kpọtụrụ aha n'elu iji wepụ mmetọ, a na-enwekwa inoculation na sterilization na ụmụ anụmanụ, ụzọ macrophage phagocytosis, ụzọ nke ịgbakwunye bromouracil na-emerụ emerụ.karama omenalana mgbe ahụ na-eji ọkụ na-enwu ha ọkụ, na ụzọ nzacha, wdg, ma ha niile na-enwe nsogbu na-adịghị arụ ọrụ.Ya mere, ozugbo mmetọ mycoplasma na-eme, ọ gwụla ma ọ bara uru karịsịa, a na-atụfu ya ma megharịa ya.

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