• lab-217043_1280

Lelee ngwa atọ nke karama ọkara PETG

Kalama omenala PETGbụ karama rọba a na-ejikarị eme ihe.Ahụ karama ya na-apụta ìhè nke ukwuu, na-anabata ime square, ịdị arọ dị arọ, ọ dịghịkwa mfe ịgbaji.Ọ bụ ezigbo akpa nchekwa.Ngwa anyị na-ahụkarị bụ atọ ndị a:

1. Serum: Serum na-enye mkpụrụ ndụ ihe ndị bụ isi na-edozi ahụ, ihe ndị na-eto eto, protein na-ejikọta, wdg, iji zere mmebi usoro nke mkpụrụ ndụ, na ichekwa sel na omenala.Ekwesịrị ịchekwa ọbara maka nchekwa ogologo oge na ọnọdụ okpomọkụ dị ala nke -20 ° C ruo -70 Celsius.Ọ bụrụ na echekwara ya na friji 4 Celsius C, n'ozuzu ọ gaghị agafe otu ọnwa.

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2.Culture medium: Omenala usoro n'ozuzu nwere carbohydrates, nitrogenous bekee, inorganic salts, vitamin na mmiri, wdg Ọ bụghị nanị na isi ihe maka inye cell oriri na ọṅụṅụ na-akwalite cell proliferation, kamakwa ndụ gburugburu ebe obibi maka cell uto na mmeputakwa. .Ebe nchekwa nchekwa nke ọkara bụ 2 ° C-8 ° C, na-echebe site na ìhè.

3. Dị iche iche reagents: Na mgbakwunye na nchekwa nke serum na omenala usoro, PETG ọkara karama nwekwara ike-eji dị ka nchekwa containers maka dị iche iche ndu reagents, dị ka buffers, dissociation reagents, ọgwụ nje, cell cryopreservation ngwọta, ntụpọ ngwọta, Uto mgbakwunye, wdg. Ụfọdụ n'ime reagents ndị a kwesịrị ịchekwa na -20 Celsius, ebe ndị ọzọ na-echekwa na ụlọ okpomọkụ.N'agbanyeghị ebe ọ bụla, karama ọkara nwere ike izute ihe nchekwa ha chọrọ.

A na-ejikarị karama ọkara PETG jide ngwọta atọ ndị a dị n'elu.Iji mee ka ikwado nlele anya nke olu nke ngwọta, enwere ọnụ ọgụgụ dị na karama ahụ.A na-eji ihe ngwọta ndị a dị n'elu mee ihe na omenala cell, na ekwesịrị ịṅa ntị na ọrụ aseptic mgbe ị na-agbakwunye ha.


Oge nzipu: Ọgọst-02-2022