Nkeerlenmeyer shake flaskbụ akpa omenala pụrụ iche maka omenala nkwusioru cell, a pụkwara iji ya dozie, jikọta na ịchekwa mgbasa ozi dị iche iche.A na-eji omenala eme ihe mgbe ị na-azụ mkpụrụ ndụ Jurkat.

Ahịrị cell Jurkat sitere na mpụta ọbara nwata nwoke dị afọ 14 ma bụrụ cell nkwusioru.Ahịrị sel ndị sitere na Jurkat enweghị mkpụrụ ndụ ihe nketa ụfọdụ adịlarị n'ụlọ akụ omenala cell.Ahịrị lymphocyte mmadụ anwụghị anwụ nke a na-ejikarị amụ nnukwu ọrịa leukemia T cell, akara T cell, na ndị na-anabata chemokine dị iche iche nwere ike ịbanye na nje virus, ọkachasị okwu HIV.Ọ nwere ọtụtụ ngwa dị iche iche na nyocha nke ndu, dị ka itinye mkpụrụ ndụ Jurkat iji mụọ M1-RNA nke ribonuclease P, yana ọmụmụ M1-RNA nke anti-MHC klas II transcriptional activator (CIITA) iji gbochie. okwu nke MHC klas II ụmụ irighiri ihe n'elu cell.

Mgbe ị na-azụ mkpụrụ ndụ Jurkat na erlenmeyer shake flasks, RPMI1640 ọkara, 10% FBS chọrọ;A na-achịkwa okpomọkụ na 37 ° C, 5% carbon dioxide, PH uru 7.2-7.4, aseptic okpomọkụ omenala.Hichapụ ma kpochapụ mmanya mmanya 75% tupu ịkwaga na bench cell ultra-clean, wepụ cell cryovial na mmiri mmiri nitrogen tank, tinye ya ozugbo na mmiri ịsa ahụ 37 Celsius C ma maa jijiji cell cell ngwa ngwa ka ọ gbazee ngwa ngwa.Mgbe ahụ, mgbe centrifugation, pipetting na ịgwakọta, wdg, a na-etinye ya na incubator cell maka ịkụ ihe.

Selụ na-enwe mmetụta karịsịa na gburugburu ebe obibi.Mgbe ị na-azụta mkpụrụ ndụ Jurkat na erlenmeyer cell shake flasks, ekwesịrị ime ịdị ọcha nkeonwe nke ọma, ekwesịrị iji reagents na-adịghị mma, na ụkpụrụ ọrụ aseptic kwesịrị ịgbaso iji zere iwebata nje bacteria na emetụta uto cell.